goat anti sin3a Search Results


odyssey imaging system  (LI-COR)
99
LI-COR odyssey imaging system
Odyssey Imaging System, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+sin3a/custom%40odyssey-imaging-system%4010%2E1074%2Fmcp%2Era120%2E002078?v=LI-COR
Average 99 stars, based on 1 article reviews
odyssey imaging system - by Bioz Stars, 2026-07
99/100 stars
  Buy from Supplier
anti rabbit igg antibody  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti rabbit igg antibody
The <t>Sin3A</t> co-repressor regulates ELK3 transcription in HUVECs. (A) The MT1-MMP promoter activity in several cell lines: 293 (human embryonic kidney cells), L-132 (human embryonic pulmonary epithelial cells) and HUVECs (human umbilical vein endothelial cells). The 1.5 kb MT1-MMP promoter was co-transfected with an empty vector (pcDNA3.1), ETS-1 or ELK3 cDNA. After 24 h, the luciferase activity was measured. (B) The protein expression levels of Sin3A in 293 cells, L-132 cells and HUVECs. The proteins were isolated from the 3 cell lines and separated by SDS-PAGE to detect <t>Sin3A.</t> <t>β-actin</t> was used as an internal control. The density of bands was measured by GelQuant.ELK3 software (lower table) (C) Confirmation of Sin3A protein expression in HUVECs following ELK3 overexpression by Western blotting. The ELK3 expression plasmid was transfected into the HUVECs. ELK3 overexpression was confirmed by Western blotting. Sin3A protein expression decreased in response to ELK3 overexpression in HUVECs. The intensity of bands was accessed by GelQuant.ELK3 software (lower table) (D) Sin3A overexpression in HUVECs almost completely abolished ELK3 expression. The intensity of bands was evaluated by GelQuant.ELK3 software (lower table). *p < 0.01 in comparison between ETS-1 and ELK3 transfected cells.
Anti Rabbit Igg Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+sin3a/pmc03979996-84-32-15?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
anti rabbit igg antibody - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
goat anti sin3a  (R&D Systems)
92
R&D Systems goat anti sin3a
The <t>Sin3A</t> co-repressor regulates ELK3 transcription in HUVECs. (A) The MT1-MMP promoter activity in several cell lines: 293 (human embryonic kidney cells), L-132 (human embryonic pulmonary epithelial cells) and HUVECs (human umbilical vein endothelial cells). The 1.5 kb MT1-MMP promoter was co-transfected with an empty vector (pcDNA3.1), ETS-1 or ELK3 cDNA. After 24 h, the luciferase activity was measured. (B) The protein expression levels of Sin3A in 293 cells, L-132 cells and HUVECs. The proteins were isolated from the 3 cell lines and separated by SDS-PAGE to detect <t>Sin3A.</t> <t>β-actin</t> was used as an internal control. The density of bands was measured by GelQuant.ELK3 software (lower table) (C) Confirmation of Sin3A protein expression in HUVECs following ELK3 overexpression by Western blotting. The ELK3 expression plasmid was transfected into the HUVECs. ELK3 overexpression was confirmed by Western blotting. Sin3A protein expression decreased in response to ELK3 overexpression in HUVECs. The intensity of bands was accessed by GelQuant.ELK3 software (lower table) (D) Sin3A overexpression in HUVECs almost completely abolished ELK3 expression. The intensity of bands was evaluated by GelQuant.ELK3 software (lower table). *p < 0.01 in comparison between ETS-1 and ELK3 transfected cells.
Goat Anti Sin3a, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+sin3a/pmc11350972-133-68-71?v=R%26D+Systems
Average 92 stars, based on 1 article reviews
goat anti sin3a - by Bioz Stars, 2026-07
92/100 stars
  Buy from Supplier
anti sin3a  (Bethyl)
91
Bethyl anti sin3a
The <t>Sin3A</t> co-repressor regulates ELK3 transcription in HUVECs. (A) The MT1-MMP promoter activity in several cell lines: 293 (human embryonic kidney cells), L-132 (human embryonic pulmonary epithelial cells) and HUVECs (human umbilical vein endothelial cells). The 1.5 kb MT1-MMP promoter was co-transfected with an empty vector (pcDNA3.1), ETS-1 or ELK3 cDNA. After 24 h, the luciferase activity was measured. (B) The protein expression levels of Sin3A in 293 cells, L-132 cells and HUVECs. The proteins were isolated from the 3 cell lines and separated by SDS-PAGE to detect <t>Sin3A.</t> <t>β-actin</t> was used as an internal control. The density of bands was measured by GelQuant.ELK3 software (lower table) (C) Confirmation of Sin3A protein expression in HUVECs following ELK3 overexpression by Western blotting. The ELK3 expression plasmid was transfected into the HUVECs. ELK3 overexpression was confirmed by Western blotting. Sin3A protein expression decreased in response to ELK3 overexpression in HUVECs. The intensity of bands was accessed by GelQuant.ELK3 software (lower table) (D) Sin3A overexpression in HUVECs almost completely abolished ELK3 expression. The intensity of bands was evaluated by GelQuant.ELK3 software (lower table). *p < 0.01 in comparison between ETS-1 and ELK3 transfected cells.
Anti Sin3a, supplied by Bethyl, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+sin3a/pmc06728282-141-46-34?v=Bethyl
Average 91 stars, based on 1 article reviews
anti sin3a - by Bioz Stars, 2026-07
91/100 stars
  Buy from Supplier
goat-anti-mouse hrp-conjugated antibodies  (Promega)
90
Promega goat-anti-mouse hrp-conjugated antibodies
The <t>Sin3A</t> co-repressor regulates ELK3 transcription in HUVECs. (A) The MT1-MMP promoter activity in several cell lines: 293 (human embryonic kidney cells), L-132 (human embryonic pulmonary epithelial cells) and HUVECs (human umbilical vein endothelial cells). The 1.5 kb MT1-MMP promoter was co-transfected with an empty vector (pcDNA3.1), ETS-1 or ELK3 cDNA. After 24 h, the luciferase activity was measured. (B) The protein expression levels of Sin3A in 293 cells, L-132 cells and HUVECs. The proteins were isolated from the 3 cell lines and separated by SDS-PAGE to detect <t>Sin3A.</t> <t>β-actin</t> was used as an internal control. The density of bands was measured by GelQuant.ELK3 software (lower table) (C) Confirmation of Sin3A protein expression in HUVECs following ELK3 overexpression by Western blotting. The ELK3 expression plasmid was transfected into the HUVECs. ELK3 overexpression was confirmed by Western blotting. Sin3A protein expression decreased in response to ELK3 overexpression in HUVECs. The intensity of bands was accessed by GelQuant.ELK3 software (lower table) (D) Sin3A overexpression in HUVECs almost completely abolished ELK3 expression. The intensity of bands was evaluated by GelQuant.ELK3 software (lower table). *p < 0.01 in comparison between ETS-1 and ELK3 transfected cells.
Goat Anti Mouse Hrp Conjugated Antibodies, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+sin3a/pmc07713351-189-28-37?v=Promega
Average 90 stars, based on 1 article reviews
goat-anti-mouse hrp-conjugated antibodies - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
goat anti-egfr antibody  (GeneTex)
90
GeneTex goat anti-egfr antibody
The <t>Sin3A</t> co-repressor regulates ELK3 transcription in HUVECs. (A) The MT1-MMP promoter activity in several cell lines: 293 (human embryonic kidney cells), L-132 (human embryonic pulmonary epithelial cells) and HUVECs (human umbilical vein endothelial cells). The 1.5 kb MT1-MMP promoter was co-transfected with an empty vector (pcDNA3.1), ETS-1 or ELK3 cDNA. After 24 h, the luciferase activity was measured. (B) The protein expression levels of Sin3A in 293 cells, L-132 cells and HUVECs. The proteins were isolated from the 3 cell lines and separated by SDS-PAGE to detect <t>Sin3A.</t> <t>β-actin</t> was used as an internal control. The density of bands was measured by GelQuant.ELK3 software (lower table) (C) Confirmation of Sin3A protein expression in HUVECs following ELK3 overexpression by Western blotting. The ELK3 expression plasmid was transfected into the HUVECs. ELK3 overexpression was confirmed by Western blotting. Sin3A protein expression decreased in response to ELK3 overexpression in HUVECs. The intensity of bands was accessed by GelQuant.ELK3 software (lower table) (D) Sin3A overexpression in HUVECs almost completely abolished ELK3 expression. The intensity of bands was evaluated by GelQuant.ELK3 software (lower table). *p < 0.01 in comparison between ETS-1 and ELK3 transfected cells.
Goat Anti Egfr Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+sin3a/pm39192337-148-60-64?v=GeneTex
Average 90 stars, based on 1 article reviews
goat anti-egfr antibody - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
odyssey  (LI-COR)
99
LI-COR odyssey
The <t>Sin3A</t> co-repressor regulates ELK3 transcription in HUVECs. (A) The MT1-MMP promoter activity in several cell lines: 293 (human embryonic kidney cells), L-132 (human embryonic pulmonary epithelial cells) and HUVECs (human umbilical vein endothelial cells). The 1.5 kb MT1-MMP promoter was co-transfected with an empty vector (pcDNA3.1), ETS-1 or ELK3 cDNA. After 24 h, the luciferase activity was measured. (B) The protein expression levels of Sin3A in 293 cells, L-132 cells and HUVECs. The proteins were isolated from the 3 cell lines and separated by SDS-PAGE to detect <t>Sin3A.</t> <t>β-actin</t> was used as an internal control. The density of bands was measured by GelQuant.ELK3 software (lower table) (C) Confirmation of Sin3A protein expression in HUVECs following ELK3 overexpression by Western blotting. The ELK3 expression plasmid was transfected into the HUVECs. ELK3 overexpression was confirmed by Western blotting. Sin3A protein expression decreased in response to ELK3 overexpression in HUVECs. The intensity of bands was accessed by GelQuant.ELK3 software (lower table) (D) Sin3A overexpression in HUVECs almost completely abolished ELK3 expression. The intensity of bands was evaluated by GelQuant.ELK3 software (lower table). *p < 0.01 in comparison between ETS-1 and ELK3 transfected cells.
Odyssey, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+sin3a/custom%40Odyssey%4010%2E1074%2Fmcp%2Era120%2E002078?v=LI-COR
Average 99 stars, based on 1 article reviews
odyssey - by Bioz Stars, 2026-07
99/100 stars
  Buy from Supplier
goatarabbit irdye 800cw  (LI-COR)
99
LI-COR goatarabbit irdye 800cw
The <t>Sin3A</t> co-repressor regulates ELK3 transcription in HUVECs. (A) The MT1-MMP promoter activity in several cell lines: 293 (human embryonic kidney cells), L-132 (human embryonic pulmonary epithelial cells) and HUVECs (human umbilical vein endothelial cells). The 1.5 kb MT1-MMP promoter was co-transfected with an empty vector (pcDNA3.1), ETS-1 or ELK3 cDNA. After 24 h, the luciferase activity was measured. (B) The protein expression levels of Sin3A in 293 cells, L-132 cells and HUVECs. The proteins were isolated from the 3 cell lines and separated by SDS-PAGE to detect <t>Sin3A.</t> <t>β-actin</t> was used as an internal control. The density of bands was measured by GelQuant.ELK3 software (lower table) (C) Confirmation of Sin3A protein expression in HUVECs following ELK3 overexpression by Western blotting. The ELK3 expression plasmid was transfected into the HUVECs. ELK3 overexpression was confirmed by Western blotting. Sin3A protein expression decreased in response to ELK3 overexpression in HUVECs. The intensity of bands was accessed by GelQuant.ELK3 software (lower table) (D) Sin3A overexpression in HUVECs almost completely abolished ELK3 expression. The intensity of bands was evaluated by GelQuant.ELK3 software (lower table). *p < 0.01 in comparison between ETS-1 and ELK3 transfected cells.
Goatarabbit Irdye 800cw, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+sin3a/custom%40926-32211%4010%2E1074%2Fmcp%2Era120%2E002078?v=LI-COR
Average 99 stars, based on 1 article reviews
goatarabbit irdye 800cw - by Bioz Stars, 2026-07
99/100 stars
  Buy from Supplier
goat anti somatostatin  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology goat anti somatostatin
The <t>Sin3A</t> co-repressor regulates ELK3 transcription in HUVECs. (A) The MT1-MMP promoter activity in several cell lines: 293 (human embryonic kidney cells), L-132 (human embryonic pulmonary epithelial cells) and HUVECs (human umbilical vein endothelial cells). The 1.5 kb MT1-MMP promoter was co-transfected with an empty vector (pcDNA3.1), ETS-1 or ELK3 cDNA. After 24 h, the luciferase activity was measured. (B) The protein expression levels of Sin3A in 293 cells, L-132 cells and HUVECs. The proteins were isolated from the 3 cell lines and separated by SDS-PAGE to detect <t>Sin3A.</t> <t>β-actin</t> was used as an internal control. The density of bands was measured by GelQuant.ELK3 software (lower table) (C) Confirmation of Sin3A protein expression in HUVECs following ELK3 overexpression by Western blotting. The ELK3 expression plasmid was transfected into the HUVECs. ELK3 overexpression was confirmed by Western blotting. Sin3A protein expression decreased in response to ELK3 overexpression in HUVECs. The intensity of bands was accessed by GelQuant.ELK3 software (lower table) (D) Sin3A overexpression in HUVECs almost completely abolished ELK3 expression. The intensity of bands was evaluated by GelQuant.ELK3 software (lower table). *p < 0.01 in comparison between ETS-1 and ELK3 transfected cells.
Goat Anti Somatostatin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+sin3a/pmc07243292-118-77-80?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
goat anti somatostatin - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
tritonx100  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology tritonx100
The <t>Sin3A</t> co-repressor regulates ELK3 transcription in HUVECs. (A) The MT1-MMP promoter activity in several cell lines: 293 (human embryonic kidney cells), L-132 (human embryonic pulmonary epithelial cells) and HUVECs (human umbilical vein endothelial cells). The 1.5 kb MT1-MMP promoter was co-transfected with an empty vector (pcDNA3.1), ETS-1 or ELK3 cDNA. After 24 h, the luciferase activity was measured. (B) The protein expression levels of Sin3A in 293 cells, L-132 cells and HUVECs. The proteins were isolated from the 3 cell lines and separated by SDS-PAGE to detect <t>Sin3A.</t> <t>β-actin</t> was used as an internal control. The density of bands was measured by GelQuant.ELK3 software (lower table) (C) Confirmation of Sin3A protein expression in HUVECs following ELK3 overexpression by Western blotting. The ELK3 expression plasmid was transfected into the HUVECs. ELK3 overexpression was confirmed by Western blotting. Sin3A protein expression decreased in response to ELK3 overexpression in HUVECs. The intensity of bands was accessed by GelQuant.ELK3 software (lower table) (D) Sin3A overexpression in HUVECs almost completely abolished ELK3 expression. The intensity of bands was evaluated by GelQuant.ELK3 software (lower table). *p < 0.01 in comparison between ETS-1 and ELK3 transfected cells.
Tritonx100, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+sin3a/10__1158_slash_0008___5472__can___18___0359-112-30-25?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
tritonx100 - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
goat anti mouse igg  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology goat anti mouse igg
The <t>Sin3A</t> co-repressor regulates ELK3 transcription in HUVECs. (A) The MT1-MMP promoter activity in several cell lines: 293 (human embryonic kidney cells), L-132 (human embryonic pulmonary epithelial cells) and HUVECs (human umbilical vein endothelial cells). The 1.5 kb MT1-MMP promoter was co-transfected with an empty vector (pcDNA3.1), ETS-1 or ELK3 cDNA. After 24 h, the luciferase activity was measured. (B) The protein expression levels of Sin3A in 293 cells, L-132 cells and HUVECs. The proteins were isolated from the 3 cell lines and separated by SDS-PAGE to detect <t>Sin3A.</t> <t>β-actin</t> was used as an internal control. The density of bands was measured by GelQuant.ELK3 software (lower table) (C) Confirmation of Sin3A protein expression in HUVECs following ELK3 overexpression by Western blotting. The ELK3 expression plasmid was transfected into the HUVECs. ELK3 overexpression was confirmed by Western blotting. Sin3A protein expression decreased in response to ELK3 overexpression in HUVECs. The intensity of bands was accessed by GelQuant.ELK3 software (lower table) (D) Sin3A overexpression in HUVECs almost completely abolished ELK3 expression. The intensity of bands was evaluated by GelQuant.ELK3 software (lower table). *p < 0.01 in comparison between ETS-1 and ELK3 transfected cells.
Goat Anti Mouse Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+sin3a/pmc05808772-349-98-103?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
goat anti mouse igg - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier
sap18  (Proteintech)
93
Proteintech sap18
The <t>Sin3A</t> co-repressor regulates ELK3 transcription in HUVECs. (A) The MT1-MMP promoter activity in several cell lines: 293 (human embryonic kidney cells), L-132 (human embryonic pulmonary epithelial cells) and HUVECs (human umbilical vein endothelial cells). The 1.5 kb MT1-MMP promoter was co-transfected with an empty vector (pcDNA3.1), ETS-1 or ELK3 cDNA. After 24 h, the luciferase activity was measured. (B) The protein expression levels of Sin3A in 293 cells, L-132 cells and HUVECs. The proteins were isolated from the 3 cell lines and separated by SDS-PAGE to detect <t>Sin3A.</t> <t>β-actin</t> was used as an internal control. The density of bands was measured by GelQuant.ELK3 software (lower table) (C) Confirmation of Sin3A protein expression in HUVECs following ELK3 overexpression by Western blotting. The ELK3 expression plasmid was transfected into the HUVECs. ELK3 overexpression was confirmed by Western blotting. Sin3A protein expression decreased in response to ELK3 overexpression in HUVECs. The intensity of bands was accessed by GelQuant.ELK3 software (lower table) (D) Sin3A overexpression in HUVECs almost completely abolished ELK3 expression. The intensity of bands was evaluated by GelQuant.ELK3 software (lower table). *p < 0.01 in comparison between ETS-1 and ELK3 transfected cells.
Sap18, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+sin3a/pmc07713351__42003_2020_1458_MOESM1_ESM-13-39-42?v=Proteintech
Average 93 stars, based on 1 article reviews
sap18 - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier

Image Search Results


The Sin3A co-repressor regulates ELK3 transcription in HUVECs. (A) The MT1-MMP promoter activity in several cell lines: 293 (human embryonic kidney cells), L-132 (human embryonic pulmonary epithelial cells) and HUVECs (human umbilical vein endothelial cells). The 1.5 kb MT1-MMP promoter was co-transfected with an empty vector (pcDNA3.1), ETS-1 or ELK3 cDNA. After 24 h, the luciferase activity was measured. (B) The protein expression levels of Sin3A in 293 cells, L-132 cells and HUVECs. The proteins were isolated from the 3 cell lines and separated by SDS-PAGE to detect Sin3A. β-actin was used as an internal control. The density of bands was measured by GelQuant.ELK3 software (lower table) (C) Confirmation of Sin3A protein expression in HUVECs following ELK3 overexpression by Western blotting. The ELK3 expression plasmid was transfected into the HUVECs. ELK3 overexpression was confirmed by Western blotting. Sin3A protein expression decreased in response to ELK3 overexpression in HUVECs. The intensity of bands was accessed by GelQuant.ELK3 software (lower table) (D) Sin3A overexpression in HUVECs almost completely abolished ELK3 expression. The intensity of bands was evaluated by GelQuant.ELK3 software (lower table). *p < 0.01 in comparison between ETS-1 and ELK3 transfected cells.

Journal: International Journal of Biological Sciences

Article Title: ELK3 Suppresses Angiogenesis by Inhibiting the Transcriptional Activity of ETS-1 on MT1-MMP

doi: 10.7150/ijbs.8095

Figure Lengend Snippet: The Sin3A co-repressor regulates ELK3 transcription in HUVECs. (A) The MT1-MMP promoter activity in several cell lines: 293 (human embryonic kidney cells), L-132 (human embryonic pulmonary epithelial cells) and HUVECs (human umbilical vein endothelial cells). The 1.5 kb MT1-MMP promoter was co-transfected with an empty vector (pcDNA3.1), ETS-1 or ELK3 cDNA. After 24 h, the luciferase activity was measured. (B) The protein expression levels of Sin3A in 293 cells, L-132 cells and HUVECs. The proteins were isolated from the 3 cell lines and separated by SDS-PAGE to detect Sin3A. β-actin was used as an internal control. The density of bands was measured by GelQuant.ELK3 software (lower table) (C) Confirmation of Sin3A protein expression in HUVECs following ELK3 overexpression by Western blotting. The ELK3 expression plasmid was transfected into the HUVECs. ELK3 overexpression was confirmed by Western blotting. Sin3A protein expression decreased in response to ELK3 overexpression in HUVECs. The intensity of bands was accessed by GelQuant.ELK3 software (lower table) (D) Sin3A overexpression in HUVECs almost completely abolished ELK3 expression. The intensity of bands was evaluated by GelQuant.ELK3 software (lower table). *p < 0.01 in comparison between ETS-1 and ELK3 transfected cells.

Article Snippet: Next, the membrane was incubated with monoclonal anti-Sin3A (Santa Cruz Biotechnology, sc-994) (1:1000 dilution), anti-ELK3 (Santa Cruz Biotechnology, sc-17860) (1:1000 dilution) or anti-β-actin antibody (1:3000 dilution) overnight at 4°C, then incubated with anti-rabbit IgG antibody (1:2000 dilution, secondary antibody against anti-Sin3A) or anti-goat IgG antibody (1:2000 dilution, secondary antibody against anti-ELK3 and anti-β-actin) for 1 h at room temperature.

Techniques: Activity Assay, Transfection, Plasmid Preparation, Luciferase, Expressing, Isolation, SDS Page, Software, Over Expression, Western Blot